Use of Transgenic Animals
System | Potential Use |
---|---|
Gene inactivation or deletion (“knockout”) | • Characterization of endogenous function(s) of gene product |
• Role of gene product in stress response, toxicity and carcinogenesis | |
• Development of disease models | |
Insertion of transgene | • Characterization of function(s) of transgene product |
• Role of transgene in activation or detoxification of xenobiotics | |
• Development of disease models | |
Promoter–Reporter (lacZ , GFP, luciferase) | • Characterization of promotor regulation |
• Definition of gene expression pattern | |
• Toxicity screens | |
Promoter–Transgene | • Defined spatiotemporal control of transgene expression |
• Role in pharmacokinetics and toxic response | |
Gene replacement (“humanization”) | |
• Characterization of function(s) of human gene product | |
• Role of polymorphic alleles in toxicity and carcinogenesis |