Standard method used to generate murine MAbs. Splenocytes from immunized mice are fused with mouse myeloma cells to generate antibody-secreting, immortalized hybridoma cells. The monoclonal antibody produced by individual hybridoma cell clones are then screened for optimal antigen binding, as defined by the complimentarity-determining regions (CDR) on the Fab portion of the antibody, as well as for the desired isotype, as defined by the Fc portion of the antibody.