Indian Journal of Human Genetics
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ORIGINAL ARTICLE
Year : 2013  |  Volume : 19  |  Issue : 2  |  Page : 144-149

Analysis of cytotoxic T-lymphocyte-associated antigen-4 and MMP-9 genes' methylation and their expression profiles with risk of non-alcoholic fatty liver disease


1 Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran
2 Department of Clinical Biochemistry, Zahedan University of Medical Sciences, Zahedan, Iran
3 Department of Internal Medicine, School of Medicine, Karman University of Medical Sciences, Karman, Iran

Correspondence Address:
Dor Mohammad Kordi Tamandani
Department of Biology, University of Sistan and Baluchestan, Zahedan, P.O. Box 98155 - 987
Iran
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Source of Support: Supported by research grants from the University of Sistan and Baluchistan and Zahedan University of Medical Sciences., Conflict of Interest: None


DOI: 10.4103/0971-6866.116106

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Objective: To investigate the effect of promoter methylation of cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) gene and matrix metalloproteinases (MMPs) on the risk of non-alcoholic fatty liver disease (NAFLD). Materials and Methods: CTLA-4 and MMP-9 promoter methylation were investigated using a methylation-specific polymerase chain reaction (MS-PCR) in blood samples taken from 80 NAFLD individuals and 95 healthy controls. The expression levels of CTLA-4 and MMP-9 were also assessed in 10 blood and 9 liver tissues mRNA samples from NAFLD patients. These cases were compared to the blood ( n = 10) samples of healthy controls with real-time quantitative reverse transcriptase PCR. Results: No significant relationship was found for methylation of CTLA-4 and MMP-9 between cases and controls. The relative expression of CTLA-4 and MMP-9 mRNA in NAFLD was not significantly different compared to healthy control samples. Conclusion: For the first time, our outcomes indicate that the methylation status of CTLA-4 and MMP-9 genes has no significant function on the process of NAFLD.


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