ORIGINAL ARTICLE |
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Year : 2013 | Volume
: 19
| Issue : 2 | Page : 144-149 |
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Analysis of cytotoxic T-lymphocyte-associated antigen-4 and MMP-9 genes' methylation and their expression profiles with risk of non-alcoholic fatty liver disease
Dor Mohammad Kordi Tamandani1, Mohammad Hashemi2, Sara Shafiepour3
1 Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran 2 Department of Clinical Biochemistry, Zahedan University of Medical Sciences, Zahedan, Iran 3 Department of Internal Medicine, School of Medicine, Karman University of Medical Sciences, Karman, Iran
Correspondence Address:
Dor Mohammad Kordi Tamandani Department of Biology, University of Sistan and Baluchestan, Zahedan, P.O. Box 98155 - 987 Iran
Source of Support: Supported by research grants from the University
of Sistan and Baluchistan and Zahedan University of Medical Sciences., Conflict of Interest: None | 1 |
DOI: 10.4103/0971-6866.116106
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Objective: To investigate the effect of promoter methylation of cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) gene and matrix metalloproteinases (MMPs) on the risk of non-alcoholic fatty liver disease (NAFLD).
Materials and Methods: CTLA-4 and MMP-9 promoter methylation were investigated using a methylation-specific polymerase chain reaction (MS-PCR) in blood samples taken from 80 NAFLD individuals and 95 healthy controls. The expression levels of CTLA-4 and MMP-9 were also assessed in 10 blood and 9 liver tissues mRNA samples from NAFLD patients. These cases were compared to the blood ( n = 10) samples of healthy controls with real-time quantitative reverse transcriptase PCR.
Results: No significant relationship was found for methylation of CTLA-4 and MMP-9 between cases and controls. The relative expression of CTLA-4 and MMP-9 mRNA in NAFLD was not significantly different compared to healthy control samples.
Conclusion: For the first time, our outcomes indicate that the methylation status of CTLA-4 and MMP-9 genes has no significant function on the process of NAFLD. |
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